LsaA, an antibiotic resistance ABCF, in complex with 70S ribosome from Enterococcus faecalis

2024-07-10 REL PDBe PDBe PDBe 2021-02-10 2021-05-05 2021-05-05 2024-07-10 German Research Foundation (DFG) WI3285/8-1 Germany Swedish Research Council 2017-03783 Sweden Swedish Research Council 2019-01085 Sweden European Regional Development Fund 2014-2020.4.01.15-0013 Sweden Estonian Research Council PRG335 Estonia Swedish Research Council 2018-00956 Sweden LsaA, an antibiotic resistance ABCF, in complex with 70S ribosome from Enterococcus faecalis Crowe-McAuliffe C Kasari M Antibiotic resistance element, ribosomal protein, LsaA, ABCF, target protection, antibiotic resistance, RIBOSOME Crowe-McAuliffe C Murina V Turnbull KJ Kasari M Mohamad M Polte C Takada H Vaitkevicius K Johansson J Ignatova Z Atkinson GC O'Neill AJ Hauryliuk V Wilson DN Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogens. Nat Commun UK 12 3577 3577 2021 34117249 doi:10.1038/s41467-021-23753-1 2041-1723 7nhk FULLOVERLAP LsaA-in complex with 70S ribosome, mRNA, and tRNA LsaA-in complex with 70S ribosome, mRNA, and tRNA 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 Enterococcus faecalis 2.2 ABC-F type ribosomal protection protein Lsa(A) Enterococcus faecalis 0.06237236299999999 1 Enterococcus faecalis LEVO MSKIELKQLSFAYDNQEALLFDQANITMDTNWKLGLIGRNGRGKTTLLRLLQKQLDYQGEILHQVDFVYFPQTVAEEQQL TYYVLQEVTSFEQWKLERELTLLNVDPEVLWRPFSSLSGGEKTKVLLGLLFIEENAFPLIDQPTNHLDLAGRQQVAEYLK KKKHGFILVSHDRAFVDEVVDHILAIEKSQLTLYQGNFSIYEEQKKLRDAFELAENEKIKKEVNRLKETARKKAEWSMNR EGDKYGNAKEKGSGAIFDTGAIGARAARVMKRSKHIQQRAETQLAEKEKLLKDLEYIDSLSMDYQPTHHKTLLTVEELRL GYEKNWLFAPISFSINAGEIVGITGKNGSGKSSLIQYLLDNFSGDSEGEATLAHQLTISYVRQDYEDNQGTLSEFAEKNQ LDYTQFLNNLRKLGMERAVFTNRIEQMSMGQRKKVEVAKSLSQSAELYIWDQPLNYLDVFNHQQLEALILSVKPAMLVIE HDAHFMKKITDKKIVLKSGHHHHHHAKGGENLYFQGVADYKDHDGDYKDHDIDYKDDDDKG A0A4U4C430 50S ribosomal protein L29 Enterococcus faecalis 0.0073425619999999995 1 LEVO MKVKEIRELTTAEMLDKEKQLKEELFNLRFQLATGQLENTARIKEVRQSIARIKTVLREQAN A0A1B4XKV0 50S ribosomal protein L30 Enterococcus faecalis 0.0063654689999999995 1 LEVO MAELKITLKRSVIGRPQNQRATVKALGLGKVNSTVTKPANEAIKGMVNTISHLVDVEEV A0A1B4XKW1 50S ribosomal protein L32 Enterococcus faecalis 0.0065487589999999995 1 LEVO MAVPARRTSKAKKAKRRTHYKLTIKGLNACSNCGEMKKSHHVCPACGHYDGKDVMSKEA A0A1B4XMM2 50S ribosomal protein L33 Enterococcus faecalis 0.006052027 1 LEVO MRVNITLECTSCKERNYLTNKNKRNNPDRLEKQKYCPRERKVTLHRETK A0A1B4XR98 50S ribosomal protein L34 Enterococcus faecalis 0.005373507 1 LEVO MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVISA A0A1B4XSI4 50S ribosomal protein L35 Enterococcus faecalis 0.007727316 1 LEVO MPKQKTHRGLAKRVKRTGGGGLKRGRAFTSHRFHGKTKKQRRQLRKASMVAKGDYKRIRQQLARMK A0A1B4XM73 50S ribosomal protein L36 Enterococcus faecalis 0.0044405339999999995 1 LEVO MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG A0A1B4XKT9 23S rRNA Enterococcus faecalis 0.9440006249999999 1 GGUUAAGUGAAUAAGGGCGCACGGUGGAUGCCUUGGCACUAGGAGCCGAUGAAGGACGGGACUAACACCGAUAUGCUUUG GGGAGCUGUAAGUAAGCUAUGAUCCAGAGAUUUCCGAAUGGGGGAACCCAAUAUCUUUUAUAGGAUAUUACUUUUCAGUG AAUACAUAGCUGAUUAGAGGUAGACGCAGAGAACUGAAACAUCUUAGUACCUGCAGGAAGAGAAAGAAAAUUCGAUUCCC UGAGUAGCGGCGAGCGAAACGGGAAGAGCCCAAACCAACAAGCUUGCUUGUUGGGGUUGUAGGACUCCGAUAUGGUAGUC UGUUAGUAUAGUUGAAGGAUUUGGAAAAUUCCGCUAAAGAGGGUGAAAGCCCCGUAGACGAAAUGCUGACAACACCUAGG AGGAUCCUGAGUACGGCGGAACACGAGAAAUUCCGUCGGAAUCCGCGGGGACCAUCCCGCAAGGCUAAAUACUCCCUAGU GACCGAUAGUGAACCAGUACCGUGAGGGAAAGGUGAAAAGCACCCCGGAAGGGGAGUGAAAUAGAUCCUGAAACCGUGUG CCUACAACAAGUCAAAGCUCGUUAAUGAGUGAUGGCGUGCCUUUUGUAGAAUGAACCGGCGAGUUACGAUUGCAUGCGAG GUUAAGUCGAAGAGACGGAGCCGCAGCGAAAGCGAGUCUGAAUAGGGCGAAUGAGUAUGUAGUCGUAGACCCGAAACCAU GUGAUCUACCCAUGUCCAGGUUGAAGGUGCGGUAAAACGCACUGGAGGACCGAACCCACGUACGUUGAAAAGUGCGGGGA UGAGGUGUGGGUAGCGGAGAAAUUCCAAACGAACUUGGAGAUAGCUGGUUCUCUCCGAAAUAGCUUUAGGGCUAGCCUCG GAAUUGAGAAUGAUGGAGGUAGAGCACUGUUUGGACUAGGGGCCCAUCUCGGGUUACCGAAUUCAGAUAAACUCCGAAUG CCAUUCAUUUAUAUCCGGGAGUCAGACUGCGAGUGAUAAGAUCCGUAGUCGAAAGGGAAACAGCCCAGACCACCAGCUAA GGUCCCAAAAUAUAUGUUAAGUGGAAAAGGAUGUGGGGUUGCACAGACAACUAGGAUGUUGGCUUAGAAGCAGCCACCAU UUAAAGAGUGCGUAAUAGCUCACUAGUCGAGUGACCCUGCGCCGAAAAUGUACCGGGGCUAAACAUAUUACCGAAGCUGU GGACUACACCAUUAGGUGUAGUGGUAGGAGAGCGUUCUAAGGGCGUUGAAGGUCGAUCGUGAGGACGGCUGGAGCGCUUA GAAGUGAGAAUGCCGGUAUGAGUAGCGAAAGACAGGUGAGAAUCCUGUCCACCGUAUGACUAAGGUUUCCUGGGGAAGGC UCGUCCGCCCAGGGUUAGUCGGGACCUAAGCCGAGGCCGAUAGGCGUAGGCGAUGGACAACAGGUUGAUAUUCCUGUACC AGUUGUUUUUGUUUGAGCAAUGGAGGGACGCAGUAGGCUAAGGAAUGCAUGCGAUUGGAAGUGCAUGUCCAAGCAAUGAG UCUUGAGUAGAGUUAAAUGCUUUACUCUUUAAGGACAAGUUGUGACGGGGAGCGAAAUAAUAGUAGCGAAGUUCCUGACG UCACACUGCCAAGAAAAGCUUCUAGUGAGAAAACAACUGCCCGUACCGUAAACCGACACAGGUAGUCGAGGAGAGUAUCC UAAGGUGAGCGAGCGAACUCUCGUUAAGGAACUCGGCAAAAUGACCCCGUAACUUCGGGAGAAGGGGUGCUGACUUCGGU CAGCCGCAGUGAAUAGGCCCAAGCGACUGUUUAUCAAAAACACAGGUCUCUGCAAAAUCGUAAGAUGAAGUAUAGGGGCU GACGCCUGCCCGGUGCUGGAAGGUUAAGAGGAUGGGUUAGCUUCGGCGAAGCUCAGAAUUGAAGCCCCAGUAAACGGCGG CCGUAACUAUAACGGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCCGCACGAAAGGCGUAACGAUUUGGG CACUGUCUCAACGAGAGACUCGGUGAAAUUUUAGUACCUGUGAAGAUGCAGGUUACCCGCGACAGGACGGAAAGACCCCA UGGAGCUUUACUGUAGUUUGAUAUUGAGUGUUUGUACCACAUGUACAGGAUAGGUAGGAGCCGAUGAGACCGGAACGCUA GUUUCGGAGGAGGCGCUGGUGGGAUACUACCCUUGUGUUAUGAACCCUCUAACCCGCACCACUAAUCGUGGUGGGAGACA GUGUCAGAUGGGCAGUUUGACUGGGGCGGUCGCCUCCUAAAAGGUAACGGAGGCGCCCAAAGGUUCCCUCAGAAUGGUUG GAAAUCAUUCGAAGAGUGUAAAGGCAGAAGGGAGCUUGACUGCGAGACCUACAAGUCGAGCAGGGACGAAAGUCGGGCUU AGUGAUCCGGUGGUUCCGCAUGGAAGGGCCAUCGCUCAACGGAUAAAAGCUACCCUGGGGAUAACAGGCUUAUCUCCCCC AAGAGUCCACAUCGACGGGGAGGUUUGGCACCUCGAUGUCGGCUCGUCGCAUCCUGGGGCUGUAGUCGGUCCCAAGGGUU GGGCUGUUCGCCCAUUAAAGCGGCACGCGAGCUGGGUUCAGAACGUCGUGAGACAGUUCGGUCCCUAUCCGUCGCGGGCG UUGGAAAUUUGAGAGGAGCUGUCCUUAGUACGAGAGGACCGGGAUGGACUUACCGCUGGUGUACCAGUUGUUCUGCCAAG GGCAUUGCUGGGUAGCUAUGUAGGGAAGGGAUAAACGCUGAAAGCAUCUAAGUGUGAAGCCCACCUCAAGAUGAGAUUUC CCAUUUCUUUAAGAAAGUAAGACCCCUGAGAGAUGAUCAGGUAGAUAGGUUGGAAGUGGAAGGCUAGUGAUAGUUGGAGC GGACCAAUACUAAUCGGUCGAGGACUUAACCA 5S rRNA Enterococcus faecalis 0.037433188 1 UGUGGUGGCGAUAGCGAGAAGGAUACACCUGUUCCCAUGCCGAACACAGAAGUUAAGCUUCUUAGCGCCGAUUGUAGUGA AGGGUUUCCCUUUGUGAGAGUAGGACGUCGCCACGC 50S ribosomal protein L2 Enterococcus faecalis 0.030291008 1 LEVO MAIKKYKPTTNGRRNMTSSDFAEITTSTPEKSLLQPLKNNAGRNNNGRITVRHQGGGHKRQYRVIDFKRNKDNVAAVVKT IEYDPNRSANIALVHYEDGVKAYILAPKGLEVGMRLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT SAQVLGKEGKYVLIRLNSGEVRMILATCRATIGSVGNEQHELINIGKAGRSRWMRKRPTVRGSVMNPNDHPHGGGEGKTP IGRKAPVSPWGQPAIGYKTRNKKAKSDKLIVRRRTK A0A1B4XKR6 50S ribosomal protein L3 Enterococcus faecalis 0.022758478999999998 1 LEVO MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTVETDGYEAIQVGYQDKREVLSNKPAKGHVAKANTAPKRFI KEFKNVELGEYEVGKEIKVDVFQAGDVVDVTGTTKGKGFQGAIKRHGQSRGPMSHGSRYHRRPGSMGPVAPNRVFKNKRL AGRMGGDRVTIQNLEVVKVDVERNVILIKGNIPGAKKSLITIKSAVKAK A0A1B4XKR3 50S ribosomal protein L4 Enterococcus faecalis 0.022549717 1 LEVO MPNVALFKQDGTQNGEITLNEEIFGIEPNESVVYDAIIMQRASLRQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRS PQWRGGGVVFGPTPRSYSYKFPKKVRRLAMKSVLSDKVAENNLVAVEGLSFDAPKTKEFKQVLANLSIDTKVLVVLENGN DFAALSARNLPNVSVVTSDNVSVLDVVSANKVLATQTALTQIEEVLA A0A4U4BZI1 50S ribosomal protein L5 Enterococcus faecalis 0.02012349 1 LEVO MNRLKEKYIKEVTPSLVEKFNYSSVMQTPKVDKIVINMGVGDAVSNAKNLDKAVEELALITGQKPLITKAKKSIAGFRLR EGMPIGAKVTLRGERMYEFLDKLVTVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA NTDEESRELLAQLGMPFQK A0A1B4XKS2 50S ribosomal protein L6 Enterococcus faecalis 0.019437303 1 LEVO MSRIGNKVVVLPAGVEIKQDGNNITVKGPKGELTREFSSDIKMNIEGNEVTFIRPNDSKEMKTIHGTTRANFNNMVVGVS EGFQKALELIGVGYRAQVQGNKLTLNVGYSHPVEMTAPEGVTFEVPANTQVIVKGINKEVVGELAANIRGVRPPEPYKGK GIRYVGEFVRRKEGKTGK A0A4U4BXV7 50S ribosomal protein L13 Enterococcus faecalis 0.016325902 1 LEVO MRTTYMAKAGEVERKWYVVDATDVPLGRLSAVVASVLRGKNKPTFTPHVDTGDFVIVINADKVKLTGKKATDKIYYRHSM YPGGLKSVTAGELRDKNSRRLIETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI A0A1B4XSB2 50S ribosomal protein L14 Enterococcus faecalis 0.013165274999999999 1 LEVO MIQQESRLRVADNSGAREILTIKVLGGSGRKTANIGDVIVATVKQATPGGVVKKGEVVKAVIVRTKSGARRADGSYIKFD ENAAVIIRDDKSPRGTRIFGPVARELRENNFMKIVSLAPEVL A0A1B4XKS1 50S ribosomal protein L15 Enterococcus faecalis 0.015602867999999999 1 LEVO MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGGGVRLGFEGGQTPLFRRLPKRGFTNINRKDYAVVNLD TLNRFEDGTEVTPVVLKEAGIVKNEKAGIKVLADGELTKKLTVKAAKFSKSAQEAIEAAGGSIEVI A0A1B4XKT1 50S ribosomal protein L16 Enterococcus faecalis 0.016261168 1 LEVO MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEWGLQATESHWITNRQIEAARIAMTRYMKRGGKVWIKIFPHKSYTSKAIG VRMGKGKGAPEGWVSPVKRGKIMFEIAGVPEEVAREALRLASHKLPVKTKIVKREEMGGESNEG A0A1B4XKU1 50S ribosomal protein L17 Enterococcus faecalis 0.014503704 1 LEVO MSYRKLGRTSSQRKAMLRDITTDLIINERIVTTEARAKEVRSTVEKMITLGKRGDLHARRQAATFVRNEVASVREEDESI VVESALQKLFNDLGPRFAERQGGYTRILKTEPRRGDAAPMVVIEFVK A0A1B4XKX1 50S ribosomal protein L18 Enterococcus faecalis 0.012937829 1 LEVO MITKPDKNKTRQKRHRRVRNKISGTAECPRLNIFRSNKNIYAQVIDDVAGVTLASASALDKEISGGTKTETAAAVGKLVA ERAAEKGIKKVVFDRGGYLYHGRVQALAEAARENGLEF A0A1B4XKS7 50S ribosomal protein L19 Enterococcus faecalis 0.013244478999999998 1 LEVO MNPLIQELTQEQLRTDIPAFRPGDTVRVHAKVVEGTRERIQLFEGVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPR VAQIEVVRYGKVRRAKLYYLRALHGKAARIKEIRR A0A1B4XPL8 50S ribosomal protein L20 Enterococcus faecalis 0.013674152 1 LEVO MARVKGGTVTRKRRKKVLKLAKGYYGSKHTLFKSAKEQVMNSYYYAFRDRRQKKRDFRKLWIARINAAARMNGLSYSKLM HGLKLAEIDINRKMLADLAVNDAAAFTALAEQAKDALSK A0A1B4XM76 50S ribosomal protein L21 Enterococcus faecalis 0.011166986 1 LEVO MYAIIKTGGKQVKVEVGQAIYVEKLNVEAGEKVVFDEVILVGGESTKVGAPTVAGATVEGTVEKHGKQKKVVTFQYKPKK HSHRKQGHRQPYTKVMIEAINA A0A1B4XMC1 50S ribosomal protein L22 Enterococcus faecalis 0.012473464 1 LEVO MSEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFTPNKSAGIIEKVLMSAVANAENNFDLDVESLVVSEAF VNEGPTMKRFRPRAKGSASPINKRTSHITVVVTEK A0A1B4XKT6 50S ribosomal protein L23 Enterococcus faecalis 0.011089021000000001 1 LEVO MELLDVIKRPVITEKSMLAMDEKKYTFEVDTRANKTLVKQAVESAFDVKVANVNILNVRPKFKRMGKYAGYTKKRRKAIV TLTEDSKEIQLFEAAE A0A1B4XKR7 50S ribosomal protein L24 Enterococcus faecalis 0.011137062999999999 1 LEVO MFVKKGDKVKVITGKDKNKEGVVLAAFPKQDKVIVEGVNVVKKHQKPNQAAPQGGILEVEAPIHVSNVMVIDPSNGEATK VAFKEVDGKKVRVSKKTGEVLDK A0A1B4XKV5 50S ribosomal protein L27 Enterococcus faecalis 0.010175571999999999 1 LEVO MLLTMNLQLFAHKKGGGSTSNGRDSESKRLGAKSADGQTVTGGSILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFER KGRDKKQVSVYPVAN A0A1B4XMB1 50S ribosomal protein L28 Enterococcus faecalis 0.007005314 1 LEVO MAKVCYFTGRKTSSGNNRSHAMNSTKRTVKPNLQKVRVLIDGKPKKVWVSTRALKSGKIERV A0A1B4XRZ8 16S rRNA Enterococcus faecalis 0.504170562 1 GAGAGUUUGAUCCUGGCUCAGGACGAACGCUGGCGGCGUGCCUAAUACAUGCAAGUCGAACGCUUCUUUCCUCCCGAGUG CUUGCACUCAAUUGGAAAGAGGAGUGGCGGACGGGUGAGUAACACGUGGGUAACCUACCCAUCAGAGGGGGAUAACACUU GGAAACAGGUGCUAAUACCGCAUAACAGUUUAUGCCGCAUGGCAUAAGAGUGAAAGGCGCUUUCGGGUGUCGCUGAUGGA UGGACCCGCGGUGCAUUAGCUAGUUGGUGAGGUAACGGCUCACCAAGGCCACGAUGCAUAGCCGACCUGAGAGGGUGAUC GGCCACACUGGGACUGAGACACGGCCCAGACUCCUACGGGAGGCAGCAGUAGGGAAUCUUCGGCAAUGGACGAAAGUCUG ACCGAGCAACGCCGCGUGAGUGAAGAAGGUUUUCGGAUCGUAAAACUCUGUUGUUAGAGAAGAACAAGGACGUUAGUAAC UGAACGUCCCCUGACGGUAUCUAACCAGAAAGCCACGGCUAACUACGUGCCAGCAGCCGCGGUAAUACGUAGGUGGCAAG CGUUGUCCGGAUUUAUUGGGCGUAAAGCGAGCGCAGGCGGUUUCUUAAGUCUGAUGUGAAAGCCCCCGGCUCAACCGGGG AGGGUCAUUGGAAACUGGGAGACUUGAGUGCAGAAGAGGAGAGUGGAAUUCCAUGUGUAGCGGUGAAAUGCGUAGAUAUA UGGAGGAACACCAGUGGCGAAGGCGGCUCUCUGGUCUGUAACUGACGCUGAGGCUCGAAAGCGUGGGGAGCAAACAGGAU UAGAUACCCUGGUAGUCCACGCCGUAAACGAUGAGUGCUAAGUGUUGGAGGGUUUCCGCCCUUCAGUGCUGCAGCAAACG CAUUAAGCACUCCGCCUGGGGAGUACGACCGCAAGGUUGAAACUCAAAGGAAUUGACGGGGGCCCGCACAAGCGGUGGAG CAUGUGGUUUAAUUCGAAGCAACGCGAAGAACCUUACCAGGUCUUGACAUCCUUUGACCACUCUAGAGAUAGAGCUUUCC CUUCGGGGACAAAGUGACAGGUGGUGCAUGGUUGUCGUCAGCUCGUGUCGUGAGAUGUUGGGUUAAGUCCCGCAACGAGC GCAACCCUUAUUGUUAGUUGCCAUCAUUUAGUUGGGCACUCUAGCGAGACUGCCGGUGACAAACCGGAGGAAGGUGGGGA UGACGUCAAAUCAUCAUGCCCCUUAUGACCUGGGCUACACACGUGCUACAAUGGGAAGUACAACGAGUCGCUAGACCGCG AGGUCAUGCAAAUCUCUUAAAGCUUCUCUCAGUUCGGAUUGCAGGCUGCAACUCGCCUGCAUGAAGCCGGAAUCGCUAGU AAUCGCGGAUCAGCACGCCGCGGUGAAUACGUUCCCGGGCCUUGUACACACCGCCCGUCACACCACGAGAGUUUGUAACA CCCGAAGUCGGUGAGGUAACCUUUUUGGAGCCAGCCGCCUAAGGUGGGAUAGAUGAUUGGGGUGAAGUCGUAACAAGGUA GCCGUAUCGGAAGGUGCGGCUGGAUCACCUCCUUUCUA 30S ribosomal protein S2 Enterococcus faecalis 0.029500634999999997 1 LEVO MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQKTVKLVDAAYDYMKNVAEEGGVALFVGTKKQAQEAI KDEAIRAGQYYVNHRWLGGTLTNWDTIQKRIARLKKINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITAKMADAFIEGNQGEDQATEELFVEET PEATSIEEIVDVVEGNNESAE A0A1B4XQD8 30S ribosomal protein S3 Enterococcus faecalis 0.024415184 1 LEVO MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIATKLADAAVSTIEIERAANRVNISIHTAKPGMVIGKGG SEVENLRKELNKLTGKRVHINIVEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK A0A1B4XKR8 30S ribosomal protein S4 Enterococcus faecalis 0.023273652 1 LEVO MSRYTGPSWKVSRRLGISLSGTGKELARRPYKPGQHGPNSRGKVSEYGMQLTEKQKLRHMYGMNERQFRTLFIKASKIKE GKHGVNFMVLLEQRLDNVVYRLGLATTRRQARQLVNHGHITVDGKRVDIPSYHVEVGQVIGVREKSQNISTIKEAVEATV GRPAFVSFDTEKLEGSFTRLPERDELYPEIDEALVVEYYNQKL A0A1B4XRV7 30S ribosomal protein S5 Enterococcus faecalis 0.017444357 1 LEVO MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNGHVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGST IPHEVIGVFGGGRILMKPAVEGSGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAALRGKS VEEIIG A0A1B4XKW0 30S ribosomal protein S6 Enterococcus faecalis 0.011621188 1 LEVO MSQDTKYEIMYIIRPNIDEEAKTALVERFDTILKDNGAEVIESKDWEKRRLAYEMNGFREGIYHIVNVTSPSTAGAINEF DRLAKINDDIIRHMIVKVEA A0A1B4XKB6 30S ribosomal protein S7 Enterococcus faecalis 0.017864625 1 LEVO MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIYNSFDIIKESTGNDPLEVFEQAMKNVMPVLEVKARRV GGSNYQVPVEVRPERRTTLGLRWVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW A0A1B4XKQ3 30S ribosomal protein S8 Enterococcus faecalis 0.014936396 1 LEVO MVMTDPIADFLTRIRNANMVKHETLEVPASKIKRDIAEILKREGFIRDVEYIEDDKQGVIRVFLKYGKNEERVITNLKRI SKPGLRAYVKADEVPKVLNGLGIAIISTSEGVITDKEARAKNIGGEVIAYVW A0A1B4XKS6 30S ribosomal protein S9 Enterococcus faecalis 0.01427148 1 LEVO MAQVQYSGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADLREVINQPFGVTETKGAYDVIVNVNGGGYAGQSGAIR HGIARALLQVDPDFRSALKRAGLLTRDARMVERKKPGLKKARKASQFSKR A0A1B4XSA2 30S ribosomal protein S10 Enterococcus faecalis 0.011731739 1 LEVO MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGADVSGPIPLPTERSLYTVIRATHKYKDSREQFEMRTHKRLIDIVNPT PKTVDALMKLDLPSGVNIEIKL A0A1B4XKR5 30S ribosomal protein S11 Enterococcus faecalis 0.013739913 1 LEVO MAAKKVSRKRRVKKNIESGVAHIHSTFNNTIVMITDTHGNALAWSSAGSLGFKGSKKSTPFAAQMAAEAATKVAMEHGLK TVDVTVKGPGSGREAAIRSLQATGLEVTAIRDVTPVPHNGCRPPKRRRV A0A1B4XKV1 30S ribosomal protein S12 Enterococcus faecalis 0.015309816999999998 1 LEVO MPTINQLVRKPRKSKVEKSDSPALNKGYNSFKKTQTNVNSPQKRGVCTRVGTMTPKKPNSALRKYARVRLSNLIEVTAYI PGIGHNLQEHSVVLLRGGRVKDLPGVRYHIVRGALDTAGVNDRKQSRSKYGTKRPKA A0A1B4XKQ7 30S ribosomal protein S13 Enterococcus faecalis 0.013595773999999998 1 LEVO MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKVLANVGVSEDVRVRDLTNEQTDAIRAEIDKLKVEGDLRREVNLNIKRL MEIGSYRGIRHRRGLPTRGQNTKNNARTRKGPTKTVAGKKK A0A1B4XKT7 30S ribosomal protein S14 type Z Enterococcus faecalis 0.007172593 1 LEVO MAKKSMIAKNKRPAKHSTQAYTRCERCGRPHSVYRKFHLCRICFRELAYKGQIPGVKKASW A0A1B4XKT0 30S ribosomal protein S15 Enterococcus faecalis 0.010668236000000001 1 LEVO MAISQERKNEIIKEYARHEGDTGSPEVQIAVLTEDINQLNEHARTHKKDHHSYRGLMKKIGHRRNLLAYLRKTDIQRYRE LIQRLGLRR A0A1B4XRW3 30S ribosomal protein S16 Enterococcus faecalis 0.01035615 1 LEVO MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLKDPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVM KKHHEAKNVKK A0A1B4XP69 30S ribosomal protein S17 Enterococcus faecalis 0.0103321 1 LEVO MTEERNQRKVYQGRVVSDKMDKTITVVVETKKNHPIYGKRMKYSKKYKAHDENNTAKVGDIVKIMETRPLSATKRFRLLE VVEEAVII A0A1B4XKS3 30S ribosomal protein S18 Enterococcus faecalis 0.009262890999999999 1 LEVO MAQQRRGGRKRRKVDYIAANHIEYIDYKDTELLKRFISERGKILPRRVTGTGAKNQRKLTIAIKRARIMGLLPFVSDEQ A0A1B4XKB3 30S ribosomal protein S19 Enterococcus faecalis 0.010586332 1 LEVO MGRSLKKGPFVDDHLMKKVEAQQGAEKKKVIKTWSRRSTIFPSFVGFTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTY RGHVADDKKTKR A0A1B4XKS0 30S ribosomal protein S20 Enterococcus faecalis 0.008972319999999999 1 LEVO MPNIESAIKRVRTSANANAKNSSQTNAMRTAIKKFEEAVAAGADNVDALYNEAVKAVDMAATKGLIHKNKANRDKIRLSK LAK A0A1B4XQJ7 tRNA-fMETi Enterococcus faecalis 0.024811794999999998 1 CGCGGGGUGGAGCAGUCAGGUAGCUCGUCGGGCUCAUAACCCGAAGGUCGUAGGUUCAAAUCCUGCCCCCGCAACCA RNA (5'-R(P*GP*GP*AP*GP*GP*UP*AP*UP*GP*AP*CP*AP*A)-3') Enterococcus faecalis 0.005617279 1 GGAGGU(N)(N)(N)(N)(N)(N)(N)AUGACAA 50S ribosomal protein L1 Enterococcus faecalis 0.02449523 1 LEVO MAKKSKKMQEALKKVDATKAYSVEEAVALAKDTNIAKFDATVEVAYKLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAK GEKAKEAEAAGADFVGDDDMVAKIQGGWFDFDVVVATPDMMATVGKLGRVLGPKGLMPNPKTGTVTMDVTKAVEEVKAGK VTYRVDKAGNIHVPIGKVSFDNEKLVENFNTINDVLLKAKPSTAKGQYIKNISVTTTFGPGIHVDQASF A0A1B4XR48 50S ribosomal protein L31 type B Enterococcus faecalis 0.010138101 1 LEVO MKQDIHPNYQPVVFMDSTTGFKFLSGSTKGSSETVEWEDGNTYPLLRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGL KDENANPDA A0A1B4XMV6 ADENOSINE-5'-TRIPHOSPHATE 0.000507181 2 ATP MAGNESIUM ION 2.4305e-05 147 MG ZINC ION 6.5409e-05 3 ZN POTASSIUM ION 3.9098e-05 29 K 1,4-DIAMINOBUTANE 8.815099999999999e-05 4 PUT singleParticle particle 7.5 Quantifoil R1.2/1.3 COPPER 200 GLOW DISCHARGE ETHANE 100 277.15 FEI VITROBOT MARK III

5s blotting.

FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 -0.7000000000000001 -2.2 130000.0 GATAN K2 SUMMIT (4k x 4k) COUNTING 1 38.0 1 EMD-0176

70S ribosome from Gram-positive organism with no factor bound in the E-site.

2.9 FSC 0.143 CUT-OFF 59262 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD RELION emd_12331.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 352 352 352 0 0 0 352 352 352 366.432 366.432 366.432 90.0 90.0 90.0 X Y Z -0.04827106 0.110437125 0.0006702298 0.0042607696 1.041 1.041 1.041 0.02 AUTHOR ::::EMDATABANK.org::::EMD-12331:::: LsaA in complex with 70S ribosome from Enterococcus faecalis. Post-processed with automatically estimated B-factor. emd_12331_additional_7.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 352 352 352 0 0 0 352 352 352 366.432 366.432 366.432 90.0 90.0 90.0 X Y Z -0.016824646 0.058828674 0.0006544411 0.0031210016 1.041 1.041 1.041 AUTHOR ::::EMDATABANK.org::::EMD-12331:::: LsaA in complex with 70S ribosome from Enterococcus faecalis. Filtered by local resolution. emd_12331_additional_6.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 352 352 352 0 0 0 352 352 352 366.432 366.432 366.432 90.0 90.0 90.0 X Y Z -0.024213137 0.07144361 0.00006850307 0.0037248537 1.041 1.041 1.041 AUTHOR ::::EMDATABANK.org::::EMD-12331:::: LsaA in complex with 70S ribosome from Enterococcus faecalis. Output from RELION Refine3D. emd_12331_additional_5.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 352 352 352 0 0 0 352 352 352 366.432 366.432 366.432 90.0 90.0 90.0 X Y Z -0.016282292 0.054030705 0.00066746783 0.0031637147 1.041 1.041 1.041 AUTHOR ::::EMDATABANK.org::::EMD-12331:::: Subclass of main volume containing distorted A-site tRNA. LsaA in complex with 70S ribosome from Enterococcus faecalis. Filtered by local resolution. emd_12331_additional_4.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 352 352 352 0 0 0 352 352 352 366.432 366.432 366.432 90.0 90.0 90.0 X Y Z -0.0154350195 0.052879646 0.00064395677 0.0029835831 1.041 1.041 1.041 AUTHOR ::::EMDATABANK.org::::EMD-12331:::: Subclass of main volume with empty A-site. LsaA in complex with 70S ribosome from Enterococcus faecalis. Filtered by local resolution. emd_12331_additional_3.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 352 352 352 0 0 0 352 352 352 366.432 366.432 366.432 90.0 90.0 90.0 X Y Z -0.08175036 0.16412875 0.00044150717 0.004414643 1.041 1.041 1.041 AUTHOR ::::EMDATABANK.org::::EMD-12331:::: Mulitbody refinement focused on the large ribosomal subunit and including most of LsaA (post-processed with automatically estimated B-factor). emd_12331_additional_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 352 352 352 0 0 0 352 352 352 366.432 366.432 366.432 90.0 90.0 90.0 X Y Z -0.09326205 0.15745038 0.00018123859 0.003234216 1.041 1.041 1.041 AUTHOR ::::EMDATABANK.org::::EMD-12331:::: Mulitbody refinement focused on the small ribosomal subunit body (post-processed with automatically estimated B-factor). emd_12331_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 352 352 352 0 0 0 352 352 352 366.432 366.432 366.432 90.0 90.0 90.0 X Y Z -0.06656857 0.14157067 0.000097010125 0.0021997227 1.041 1.041 1.041 AUTHOR ::::EMDATABANK.org::::EMD-12331:::: Mulitbody refinement focused on the small ribosomal subunit head (post-processed with automatically estimated B-factor). emd_12331_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 374.76 374.76 374.76 90.0 90.0 90.0 X Y Z -0.059439894 0.11318496 0.000046205805 0.007622038 1.041 1.041 1.041 AUTHOR ::::EMDATABANK.org::::EMD-12331:::: LsaA in complex with 70S ribosome from Enterococcus faecalis. Half map 2. emd_12331_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 360 360 360 0 0 0 360 360 360 374.76 374.76 374.76 90.0 90.0 90.0 X Y Z -0.05467663 0.11894711 0.00004866052 0.0076238434 1.041 1.041 1.041 AUTHOR ::::EMDATABANK.org::::EMD-12331:::: LsaA in complex with 70S ribosome from Enterococcus faecalis. Half map 1.