REL PDBe PDBe PDBe 2006-11-25 2006-12-06 2007-12-06 2012-10-24 Electron cryotomography reveals the portal in the herpesvirus capsid. Chang JT Schmid MF Rixon FJ Chiu W Chang JT Schmid MF Rixon FJ Chiu W Electron cryotomography reveals the portal in the herpesvirus capsid. J.VIROL. 81 2065 2068 2007 17151101 doi:10.1128/JVI.02053-06 Herpes Simplex Virus 1 Herpes Simplex Virus 1 1 Human herpesvirus 1 Human herpesvirus 1 Homo sapiens INVERTEBRATES Capsid 1250 16 VIRION SPECIES false true Herpes Simplex subtomogramAveraging 7.2
170mM NaCl, 3.4mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, 6.8mM CaCl2, 4.9mM MgCl2
200 mesh copper
 ETHANE 100 293 OTHER
Vitrification instrument: Vitrobot
 JEOL 2010F FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2 3.0 3.0 20000.0 27680.0 GATAN LIQUID NITROGEN 93 93 93
Tilted from -70 to +70 in 2 degree increments using Mr T software (unpublished).
GENERIC GATAN 50 Side entry liquid nitrogen cooled 70 70
Averaged 33 particles extracted from the reconstruction. Average number of tilts used in the 3D reconstructions: 71. Average tomographic tilt angle increment: 2.
OTHER 57.0 FSC 0.5 CUT-OFF IMOD
Icosahedrally averaged
 emd_1306.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 288 288 288 -144 -144 -144 288 288 288 1563.84 1563.84 1563.84 90 90 90 X Y Z -0.117449 0.204111 -0.0153244 0.047453 5.43 5.43 5.43 0.123 This is the icosahedrally-averaged map from 33 particles.
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