2024-11-06 REL PDBe PDBe PDBe 2021-10-16 2022-07-27 2022-07-27 2024-11-06 Other private United States Human GYS1-GYG1 complex inhibited-like state bound to glucose-6-phosphate McCorvie TJ Shrestha L Glycogen, Complex, Phosphorylation, TRANSFERASE McCorvie TJ Loria PM Tu M Han S Shrestha L Froese DS Ferreira IM Berg AP Yue WW Molecular basis for the regulation of human glycogen synthase by phosphorylation and glucose-6-phosphate. Nat.Struct.Mol.Biol. US 29 628 638 2022 35835870 doi:10.1038/s41594-022-00799-3 1545-9985 EMD-13743 other EM volume
Human GYS1-GYG1 complex inhibited state
 7q0s FULLOVERLAP Ternary complex of phosphorylated full-length human glycogen synthase 1 in complex with minimum region of human glycogenin-1 Ternary complex of phosphorylated full-length human glycogen synthase 1 in complex with minimum region of human glycogenin-1 0 1 2 3 Homo sapiens muscle 0.35 Glycogen [starch] synthase, muscle Homo sapiens Muscel 0.08388542999999998 2 Spodoptera frugiperda LEVO MPLNRTLSMSSLPGLEDWEDEFDLENAVLFEVAWEVANKVGGIYTVLQTKAKVTGDEWGDNYFLVGPYTEQGVRTQVELL EAPTPALKRTLDSMNSKGCKVYFGRWLIEGGPLVVLLDVGASAWALERWKGELWDTCNIGVPWYDREANDAVLFGFLTTW FLGEFLAQSEEKPHVVAHFHEWLAGVGLCLCRARRLPVATIFTTHATLLGRYLCAGAVDFYNNLENFNVDKEAGERQIYH RYCMERAAAHCAHVFTTVSQITAIEAQHLLKRKPDIVTPNGLNVKKFSAMHEFQNLHAQSKARIQEFVRGHFYGHLDFNL DKTLYFFIAGRYEFSNKGADVFLEALARLNYLLRVNGSEQTVVAFFIMPARTNNFNVETLKGQAVRKQLWDTANTVKEKF GRKLYESLLVGSLPDMNKMLDKEDFTMMKRAIFATQRQSFPPVCTHNMLDDSSDPILTTIRRIGLFNSSADRVKVIFHPE FLSSTSPLLPVDYEEFVRGCHLGVFPSYYEPWGYTPAECTVMGIPSISTNLSGFGCFMEEHIADPSAYGIYILDRRFRSL DDSCSQLTSFLYSFCQQSRRQRIIQRNRTERLSDLLDWKYLGRYYMSARHMALSKAFPEHFTYEPNEADAAQGYRYPRPA SVPPSPSLSRHSSPHQSEDEEDPRNGPLEEDGERYDEDEEAAKDRRNIRAPEWPRRASCTSSTSGSKRNSVDTATSSSLS TPSEPLSPTSSLGEERN P13807 2.4.1.11 Glycogen [starch] synthase, muscle Homo sapiens Muscle 0.08396540599999999 2 Spodoptera frugiperda LEVO MPLNRTLSMSSLPGLEDWEDEFDLENAVLFEVAWEVANKVGGIYTVLQTKAKVTGDEWGDNYFLVGPYTEQGVRTQVELL EAPTPALKRTLDSMNSKGCKVYFGRWLIEGGPLVVLLDVGASAWALERWKGELWDTCNIGVPWYDREANDAVLFGFLTTW FLGEFLAQSEEKPHVVAHFHEWLAGVGLCLCRARRLPVATIFTTHATLLGRYLCAGAVDFYNNLENFNVDKEAGERQIYH RYCMERAAAHCAHVFTTVSQITAIEAQHLLKRKPDIVTPNGLNVKKFSAMHEFQNLHAQSKARIQEFVRGHFYGHLDFNL DKTLYFFIAGRYEFSNKGADVFLEALARLNYLLRVNGSEQTVVAFFIMPARTNNFNVETLKGQAVRKQLWDTANTVKEKF GRKLYESLLVGSLPDMNKMLDKEDFTMMKRAIFATQRQSFPPVCTHNMLDDSSDPILTTIRRIGLFNSSADRVKVIFHPE FLSSTSPLLPVDYEEFVRGCHLGVFPSYYEPWGYTPAECTVMGIPSISTNLSGFGCFMEEHIADPSAYGIYILDRRFRSL DDSCSQLTSFLYSFCQQSRRQRIIQRNRTERLSDLLDWKYLGRYYMSARHMALSKAFPEHFTYEPNEADAAQGYRYPRPA (SEP)VPPSPSLSRHSSPHQSEDEEDPRNGPLEEDGERYDEDEEAAKDRRNIRAPEWPRRASCTSSTSGSKRNSVDTATS SSLSTPSEPLSPTSSLGEERN P13807 2.4.1.11 Glycogenin-1 Homo sapiens 0.039422621 4 Spodoptera frugiperda LEVO MTDQAFVTLTTNDAYAKGALVLGSSLKQHRTTRRLVVLATPQVSDSMRKVLETVFDEVIMVDVLDSGDSAHLTLMKRPEL GVTLTKLHCWSLTQYSKCVFMDADTLVLANIDDLFDREELSAAPDPGWPDCFNSGVFVYQPSVETYNQLLHLASEQGSFD GGDQGILNTFFSSWATTDIRKHLPFIYNLSSISIYSYLPAFKVFGASAKVVHFLGRVKPWNYTYDPKTKSVKSEAHDPNM THPEFLILWWNIFTTNVLPLLQQFGLVKDTCSYVNVLSDLVYTLAFSCGFCRKEDVSGAISHLSLGEIPAMAQPFVSSEE RKERWEQGQADYMGADSFDNIKRKLDTYLQ P46976 2.4.1.186 6-O-phosphono-alpha-D-glucopyranose 0.000260136 4 G6P singleParticle particle 0.75 7.5 25.0 HEPES HEPES 200.0 NaCl Sodium chloride 2.0 TCEP TCEP 0.05 Tween-20 Tween-20 5.0 Glucose-6-Phosphate Glucose-6-Phosphate
25 mM HEPES, pH 7.5, 200 mM NaCl, 2.0 mM TCEP, 0.05% (v/v) tween-20 filtered sterilised with 5 mM glucose-6-phosphate
 Quantifoil R1.2/1.3 GOLD 200 CARBON HOLEY ARRAY 12.0 PLASMA CLEANING ETHANE 100 277 FEI VITROBOT MARK II FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 100.0 2.7 0.8 2.3000000000000003 81000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K3 (6k x 4k) 1 1.22 55.0 1 4391867 D2 FOURIER SPACE 4.0 FSC 0.143 CUT-OFF RELION 3.1.1 40062 ANGULAR RECONSTITUTION RELION 3.1.1. ANGULAR RECONSTITUTION RELION 3.1.1. RELION 3.1.1. emd_13751.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 325.8 325.8 325.8 90.0 90.0 90.0 X Y Z -0.11304499 0.19776459 0.00012163403 0.004804662 1.086 1.086 1.086 0.0281 AUTHOR Locally filtered map 4QLB A 1-655 PDB experimental model 4QLB E 268-301 PDB experimental model FLEXIBLE FIT correlation coefficient REAL 81.359999999999999 emd_13751_msk_1.map emd_13751_additional_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 325.8 325.8 325.8 90.0 90.0 90.0 X Y Z -0.010151489 0.04234209 0.000055915818 0.0016303445 1.086 1.086 1.086 AUTHOR Non-sharpened map emd_13751_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 325.8 325.8 325.8 90.0 90.0 90.0 X Y Z -0.056825075 0.10901954 0.00017479876 0.0025530697 1.086 1.086 1.086 AUTHOR Sharpened map emd_13751_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 325.8 325.8 325.8 90.0 90.0 90.0 X Y Z -0.018748838 0.05018559 0.0000509136 0.0024742144 1.086 1.086 1.086 AUTHOR Half-map 1 emd_13751_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 325.8 325.8 325.8 90.0 90.0 90.0 X Y Z -0.017569954 0.049486816 0.00006117106 0.002380294 1.086 1.086 1.086 AUTHOR Half-map 2